Test Tool — Reconstitution

Lyophilization (freeze-drying) removes water from a peptide solution under vacuum, leaving a stable dry powder. Lyophilized peptides are far more stable than liquids—they can be stored at room temperature for months or frozen for years without significant degradation.

Inject bacteriostatic water slowly down the inside wall of the vial—never squirt directly onto the powder. Gently swirl or roll the vial until the powder is fully dissolved. Do not shake vigorously, as mechanical agitation can denature peptide chains and reduce potency.

Aim for a concentration that yields a convenient injection volume of 10–50 units (0.1–0.5 mL) on a 100-unit insulin syringe. Very dilute solutions mean larger injection volumes; very concentrated solutions make small doses difficult to measure accurately.

Cloudiness can indicate incomplete dissolution, particulate contamination, or protein aggregation. Allow more time for the peptide to dissolve with gentle swirling. If cloudiness persists or flakes are visible, do not use the vial—this may indicate degradation or contamination.