How to Use This Calculator
Enter the milligrams of each peptide in the vial, then the bacteriostatic water added in mL. Enter the combined amount to draw in mcg and pick the syringe size, then click Calculate. The result shows the blend concentration, the draw in mL and units, how many mcg of each peptide are in that draw, and how many doses of that size the vial holds.
Why a Blend Needs Different Maths
In a blend vial every peptide shares the same liquid, so one draw contains all of them in the ratio they were mixed. The concentration that sets the draw volume is the combined concentration, not any single peptide’s. That is why the amount here is a combined figure in mcg, and why the per-peptide table matters: a draw sized by the total can contain very different amounts of each component depending on the ratio in the vial. Always read the ratio off the vial label rather than assuming one.
Where This Fits Alongside Other Research Tools
The Peptide Reconstitution Calculator works out how much bacteriostatic water to add to reach a target concentration before the blend is mixed. The KLOW Blend Calculator covers a four-peptide blend that also contains KPV, and the BPC-157 TB-500 Blend Calculator covers the two-peptide version.
Who Should Use This
Researchers working with a mixed GLOW vial who need the syringe draw for a combined amount, and the per-peptide breakdown of that draw.
What This Calculator Does
The GLOW Blend Calculator turns the milligrams of GHK-Cu, BPC-157 and TB-500 in a vial, the bacteriostatic water added and a combined amount in mcg into the blend concentration, the draw in mL and units on a U-100 syringe, the amount of each peptide in that draw, and the number of doses the vial holds.
Formula Used
Total (mg) = GHK-Cu + BPC-157 + TB-500Concentration (mg/mL) = Total (mg) / BAC water (mL)Draw (mL) = Amount (mg) / ConcentrationUnits to draw = Draw (mL) × 100 (U-100 syringe)Each peptide in the draw (mcg) = (that peptide's mg / Total mg) × Amount (mcg)Doses per vial = FLOOR(Total (mg) / Amount (mg))Example Calculation
Limitations
This is a unit-conversion tool, not guidance on what to use. It assumes the whole vial dissolves evenly and that the ratio on the label is accurate, and it ignores dead space and draw loss. Very small draws are hard to measure on a 1 mL syringe, so a smaller syringe or a larger water volume may be needed. Confirm the contents of the vial against the supplier label before entering them.
Frequently Asked Questions
What is in a GLOW blend vial?
GLOW vials are usually sold as a mix of GHK-Cu, BPC-157 and TB-500, but the milligrams of each differ between suppliers. This calculator does not assume any ratio: you enter the figures printed on the vial or the certificate of analysis, and it works from those.
How do I work out the concentration of a blend?
Add the milligrams of every peptide in the vial, then divide by the millilitres of bacteriostatic water added. A 70 mg vial reconstituted with 2 mL is 35 mg/mL. That combined concentration is what sets the draw volume.
How much of each peptide is in one draw?
Each peptide keeps its share of the blend. Divide that peptide's milligrams by the total milligrams, then multiply by the combined amount drawn. In a 70 mg vial holding 50 mg of GHK-Cu, a 1,000 mcg draw contains 714.3 mcg of GHK-Cu.
Why is my draw less than one unit?
Blend vials are often concentrated, so a small combined amount can land below the smallest marking on a 1 mL syringe. Adding more bacteriostatic water lowers the concentration and makes the draw larger and easier to read, and the Peptide Reconstitution Volume Optimizer shows which water volume puts the draw on a clear marking.